Understanding
Cell Line Development

Many breakthroughs in research and medicine start with reliable cell lines. Precise cell line development (CLD) is key to establishing a foundation for lasting success. Choosing the right tools is the first step toward ensuring reproducible and compliant cell line development processes.

Frequently Asked Questions (FAQ)

What is cell line development?

Cell line development is the process of creating stable populations of cells with consistent genetic and phenotypic characteristics. This involves isolating single cells, expanding them into clones, and verifying their growth and function to produce reliable research or production cell lines.
Stable cell lines are critical for reproducible experiments, biomanufacturing, and therapeutic development. They ensure consistency across studies and batches, reduce variability, and support high-quality biologics production and drug discovery workflows.
Cell line development is used to generate consistent cell populations for drug discovery, biologics production, gene editing studies, and regenerative medicine research. Automated workflows accelerate these applications, enabling higher throughput and reliable experimental outcomes.
Our offerings achieve 99.99% probability of clonal derivation and generate monoclonality reports, making it simple to provide proof of clonality to regulatory bodies.

How we support cell line development

We provide lab instruments that automates, streamlines and standardizes the critical steps in cell line development, from single-cell isolation and clone verification to sterile expansion.

By automating CLD using CYTENA’s solutions, researchers can overcome key challenges such as timeline, budget, and proof of monoclonality. Our solutions enable the gentle handling of sensitive cells, ensure monoclonality, and support low-risk and regulatory compliant workflows across the entire cell line development process.

Better clones, faster

The more clones you isolate and screen, the bigger the selection to choose your best clones from. However, isolating, tracking and screening hundreds if not thousands of cells is a daunting time- and labor-intensive task when done manually. This stretches project timelines and budgets while making the process susceptible to manual errors in sample handling and documentation.

By automating single-cell dispensing and clone tracking with the UP.SIGHT by CYTENA, CLD scientists can isolate a thousand single cells in under 30 minutes, without hassle.

Automation-friendly clone characterization

The more clones, the more time- and resource-intensive their characterization is. Any compromise on characterization assays risks introducing variability and experimental noise that can jeopardize the identification of the best performing clones.

F.QUANT titer assays enable sensitive and highly reproducible titer measurement, directly from as little as 5uL culture supernatant, without cell removal or wash necessary, making the assay easily scalable to hundreds of clones, and automatable on virtually any liquid handler.

Clone-centric data management

The classic lab notebook and Laboratory Information Management Systems (LIMS) can fall short of features or convenience when it comes to keeping track of clones from multiple CLD campaigns, across multiple plates and through various characterization assays at different paces. 

C.STUDIO by CYTENA is a clone-centric, purpose-built software for CLD campaigns management. The data of all your clones from all your CLD campaigns is conveniently centralized, including Day 0 single-cell imaging, outgrowth tracking, titer and characterization assay results. The software also generates regulator-ready monoclonality reports.

Sub-Applications

Cell line development is fundamental to advancing biologics, cell-based therapies, and microbial production.

Monoclonal Antibody Production

The production of monoclonal antibodies relies on the development of stable CHO cell lines. BICO technologies accelerate this process by ensuring documented monoclonality and enabling efficient screening of high-yield clones, reducing timelines while supporting GMP-compliant production.

Cell Therapies and Viral Vector Development

Engineered or patient-derived eukaryotic cell lines are essential for CAR-T therapies and viral vector manufacturing. With precise transfection, reliable clonal tracking, and scalable expansion, BICO technologies help researchers and manufacturers meet the requirements of clinical-grade workflows.

Industrial Microbial Bioproduction

Microbial strains, such as yeast and bacteria, are utilized in biomanufacturing for the production of enzymes, metabolites, and biologics. BICO platforms enable single-cell isolation and scalable culture expansion, safeguarding genetic stability and ensuring consistent yields in industrial fermentation processes.
Microbiome research

Products for Cell Line Development

BICO offers a comprehensive suite of advanced instrumentation to enable accurate and compliant cell line development workflows. Our devices are designed to meet diverse client needs, including BSL requirements, use of rare cell types, limited working volumes, and staining protocols.

C.STATION

Automate your cell line development with precision and speed

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UP.SIGHT

Single-cell dispensing with imaging for clonality assurance
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C.SIGHT

Optimized for affordable cell line development

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F.SIGHT

Optimized for rapid dispensing of fluorescent cells

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Contact Us

Get in touch with a BICO expert today to discuss your research goals, explore our innovative solutions, and find the approach that perfectly aligns with your lab’s needs. Whether you’re looking for a quote, a product demo, or technical guidance, our team is here to support you every step of the way.
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