Business area

Biosciences

Providing user-friendly instruments to bring efficiency and speed to multiple application areas.

Smarter Workflows in:
Multiomics
Cell Line Development
Next-Generation Sequencing
Combinatorial Screenings

Bringing efficiency and speed

Molecular biology and multiomics are fast-growing fields with ever increasing requirements for higher throughput and automation. The Biosciences business area provide user-friendly instruments that allow for the gentle handling of cells and rare samples, rapid transfer of liquids and reagents as well as downstream analysis platforms to gain valuable insights from experiments, upright and inverted microscopy. Bringing efficiency and speed to multiple application areas.

Pioneering non-contact liqiud handling

The Biosciences business area consists of five industry driving companies, DISPENDIX, a pioneer in the non-contact liquid handling industry enabling highly multiplexed assembly of thousands of chemical reactions within minutes and more flexibility in assay development.

Proven single-cell dispensing tehcnology

CYTENA provides pharmaceutical companies with proven single-cell dispensing technology for the deterministic single- cell isolation of a wide range of cell types, including CHO, HEK, primary cells, yeast and bacteria. 

Complimenting product portfolio

Additionally, CYTENA has developed solutions for the automated monitoring of cell-based assays, through an innovative live-cell imaging system, complimenting Discover Echo’s portfolio of inverted and upright microscopes. Additionally, CYTENA Bioprocess Solutions supports the cell line development industry with first of their kind high-throughput microbioreactors for parallel cultivations in 96-well plates to bring production bioreactor conditions to the 150 to 800 μL scale level. 

Software integrating workflows

Most recently, Biosero has joined the Bioscience business area. As one of the leaders in developing innovative software solutions that integrate end-to-end workflows that multiply productivity in the lab there are many additional synergies to capture.

Featured Product Offering

C.BIRD™

The C.BIRD microbioreactor from CYTENA offers parallel cultivations in 96-well plates, bringing the production capacity of bioreactors to the 150 to 1,600 μL microbioreactor scale. It screens and monitors valuable cell culture parameters for hundreds of cell lines from the earliest stages, enables the early identification of the highest producing and most stable clones in order to reduce later passaging steps and scale-up efforts. Compared to traditional static culturing, the C.BIRD improves cell proliferation, recombinant protein yield and volume specific productivity

Revolution

The first of its kind, the Revolution from Discover Echo offers researchers both an inverted and upright microscope capable of ultra fast scanning, mosiac image stitching providing users with brightfield, fluorescence and phase contrast imaging capabilities. Revolution is driven by Discover Echo’s propietary software module on a Surface Studio bringing a more collaborative and intuitive experience to the user by eliminating the need for eyepieces.

UP.SIGHT™

The UP.SIGHT streamlines cell line development (CLD) workflows by automating labor-intensive and timeconsuming steps. Thanks to the combination of a patented, highly efficient, fast and gentle single-cell dispensing technology with a superfast, high-quality imaging system users acquire full well images without the need for image stitching. This all-in-one solution from CYTENA enables nozzle imaging and 3D Full Well Imaging for double assurance of clonality from two independent optical apparatuses, leading to a probability of clonality >99.99%.

S.NEST

A high-throughput microbioreactor with CO2 incubator functions. The S.NEST from CYTENA shortens the process time for cell upscaling, provides a better microscale environment
for cell growth, and brings more efficiency to cell line selection.

I.DOT

Immediate Drop-on-demand Technology I.DOT from DISPENDIX brings intuitive automation, precision and speed to non-contact liquid handling tasks like next-generation sequencing library prep, high-throughput screening and cellular assays. It reduces hands-on time and operator bias and works with low volumes for assay miniaturization. Droplets of multiple liquid classes are dispensed into the target plate below the source plate, eliminating carryover and cross-contamination.

Green Button Go Orchestrator

Green Button Go Orchestrator software suite from Biosero is an end-to-end laboratory management solution for designing, scheduling, launching, and managing lab workflows within a lab, across labs, or at facilities around the world.

Green Button Go Scheduler

Green Button Go Scheduler lets scientists integrate, control, and schedule multiple instruments and devices in the lab from a single interface, matching laboratory automation to science, creating a cohesive technology ecosystem that accelerates operations and increases productivity.

Customer Spotlights

Precise and sensitive tools to COVID-19 monitoring
Given the challenges of combating the COVID-19 pandemic, scientists in both research and diagnostic environments are looking to optimize their workflows for crucial time and cost savings.  

An influential example is a study conducted by Dr. Nicola Crosetto’s lab at Karolinska Institutet, in which the I.DOT (Immediate Drop-on-demand Technology) liquid handler was used to automate and streamline a versatile technique (COVseq) for preparing multiplex DNA sequencing libraries from low-input samples with high accuracy, speed and significant reductions in liquid reagent volumes.

The study revealed that the COVseq technique can be easily applied to ongoing pandemic genomic surveillance and adapted to monitor other pathogens such as influenza viruses. In addition, an analysis of costs showed that the technique could be used to sequence thousands of samples per week at less than $10 per sample, including library preparation and sequencing costs.

An influential example is a study conducted by Dr. Nicola Crosetto’s lab at Karolinska Institutet, in which the I.DOT (Immediate Drop-on-demand Technology) liquid handler was used to automate and streamline a versatile technique (COVseq) for preparing multiplex DNA sequencing libraries from low-input samples with high accuracy, speed and significant reductions in liquid reagent volumes.

The study revealed that the COVseq technique can be easily applied to ongoing pandemic genomic surveillance and adapted to monitor other pathogens such as influenza viruses. In addition, an analysis of costs showed that the technique could be used to sequence thousands of samples per week at less than $10 per sample, including library preparation and sequencing costs.

Read more on cellink.com →

“We were able to easily create new workflows for PCR and NGS applications and I highly recommend this system for all labs running high-throughput assays that require complex liquid dispensing schemes, as well as those aiming to lower assay costs by reducing reagent volumes.”

 

Nicola Crosetto, PhD

Automation and protocol optimization of single-cell cloning of iPS cells
Cell line development is still heavily based on “trial and error” methods and can be often laborious and time-consuming. Some of the biggest challenges include clone stability and the production of unintended molecules. The optimization of the method and the implementation of automation in the process can save precious time and resources.
Using the CYTENA UP.SIGHT, Dr. Rafal Krol optimized and automated processes in single-cell cloning and cell line development stability. The purpose was to produce monoclonal antibodies, viral vectors and iPSC cloning for cell therapy.

Using the CYTENA UP.SIGHT, Dr. Rafal Krol optimized and automated processes in single-cell cloning and cell line development stability. The purpose was to produce monoclonal antibodies, viral vectors and iPSC cloning for cell therapy.

 

Read full story on cytena.com →

“With the nozzle images, we can reliably see for each cell being dispensed, the size and roundness and we have this [information] before we start dispensing… That’s really very beneficial for the UP.SIGHT and I can say that this technological advancement is really huge.”

 

Dr. Rafal Krol
Chief Research Scientist CiRA Foundation